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pound1pastor (#836) 365 days ago
The hippocampi have been dissected from the remaining brain tissue. For dissociation, hippocampi were incubated with two.5 trypsin and mechanically dissociated by pipetting. Cell count was determined and corrected towards the desired cell density. Main hippocampal neurons had been maintained in Neurobasal media containing B27 supplement at 37 , five CO2, and 10 O2. The medium was changed 45 min