1
roastokra73 (#754) 183 days ago
N and BACE1-cterm into phCMV3 (Genlantis). An untagged SorLA construct was generated by cutting EGFP cDNA out of your SorLA-GFP vector by utilizing the flanking AgeI cleavage web pages. A quit codon was inserted by utilizing the Speedy Alter II site-directed mutagenesis kit (Stratagene, La Jolla, CA). EGFP cDNA was inserted amongst the propeptide along with the Vps10p sequences of SorLA cDNA to ge